The data set is available as an Excel file with separate tabs for spectra (wavelength, absorbance) showing the affect on the spectrum of a change in slit width, scan rate, and response time. Representative plots of the data are shown below.

File: BenzeneSpectra

Tab 1: SlitWidth

slit widths: 0.25, 1.0, 2.0, and 4.0 nm

scan rate: 20 nm/min

response: 1

Tab 2: ScanRate

slit width: 1.0 nm

scan rates: 5, 10, 20, 60, 120, 200, 300, 750, and 1500 nm/min

response: 3

Tab 3: Response

slit width: 1.0 nm

scan rate: 60 nm/min

response: 1, 2, 3, 4, 5, 6, 7

The response settings likely are as follows (from Lambda 2 spectrophotometer):

1 = 0.1 s; 2 = 0.2 s; 3 = 0.5 s; 4 = 1.0 s; 5 = 2.0 s; 6 = 5.0 s; 7 = 10.0 s

One use of the data is shown in the following histogram and superimposed normal distribution curve assuming that μ and σ^{2} for the population are equivalent to *X* and *s*^{2} for the sample. The total area of the histogram’s bars and the area under the normal distribution curve are equal.

y = 3.00 + 0.500x

correlation coefficient of 0.816

A visual examination of the data sets and their respective regression lines shows that a linear model is inappropriate for all but one of the data sets. The Excel file in this post contains the four data sets, each on a separate tab.

]]>**File: ****RiboflavinHPLC**

Tab 1: Fluorescence (time, % fluorescence)

Tab 2: UVVis (time, absorbance)

The following figure provides one presentation of the data that demonstrates the difference between the selectivity of the two detectors. The peak marked with the red asterisk is riboflavin.

Data provided by Jason Schultz, Jonna Berry, Kaelene Lundstrom, and Dwight Stoll, Department of Chemistry, Gustavus Adolphus College.

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