Tag Archives: Lineweaver-Burke

A Comprehensive Enzyme Kinetic Exercise for Biochemistry

The kinetics of the dephosphorylation of sodium p-nitrophenol using the enzyme bovine alkaline phosphatase is studied by measuring the reaction’s velocity spectrophotometrically as a function of the substrate’s concentration. The kinetic parameters Km and Vm are determined using Lineweaver-Burke and … Continue reading

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