Category Archives: Spectroscopy

Neocuproine Method: Analysis of Procedure

One method for determining copper is the “Neocuproine Method”. In this method, copper in a +1 oxidation state reacts with neocuproine (2,9-dimethyl-1,10-phenanthroline) to form a complex of Cu(neocuproine)2.  The complex is extracted into a chloroform-methanol mixture, giving a yellow solution … Continue reading

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Standards and Samples

The spectrophotometric methods for analyzing Mn and for analyzing glucose use a chemical reaction to produce a useful signal. In the analysis for Mn in steel, colorless Mn2+ is oxidized to give the purple MnO4– ion, while in the analysis for … Continue reading

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Standards for the Spectrophotometric Analysis of Acetone

Suppose you need to prepare a set of calibration standards for the spectrophotometric analysis of acetone at a wavelength of 211 nm using a 1.00 cm cell. The molar absorptivity for acetone at this wavelength is 562. To maintain an … Continue reading

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