Category Archives: Kinetic Method

Kinetic Determination of Ascorbic Acid

One method for determining the concentration of glucose in fruit juices is to take advantage of its enzymatic oxidation in the presence of glucose oxidase: β-D-glucose + O2 + H2O → δ-D-gluconolactone + H2O2 To follow the kinetics of this … Continue reading

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Kinetic Method for Manganese

Trace levels of manganese can be determined by measuring its catalytic effect on the oxidation of diethylamine by sodium periodate.  The rate of formation of the oxidation product is followed spectrophotometrically.  A series of external standards are prepared by adding … Continue reading

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Standardization of a Kinetic Method of Analysis

Shown below is a plot of initial rate vs. substrate concentration for an enzyme-substrate reaction that follows Michaelis-Menton kinetics. An analyst developing a quantitative method for the substrate wishes to use a one-point standardization. The standard selected by the analyst, … Continue reading

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