Standardization of a Kinetic Method of Analysis

Shown below is a plot of initial rate vs. substrate concentration for an enzyme-substrate reaction that follows Michaelis-Menton kinetics. An analyst developing a quantitative method for the substrate wishes to use a one-point standardization. The standard selected by the analyst, and the corresponding initial rate, are shown on the diagram. Will this one-point standardization give accurate results? Be sure to clearly justify your response.

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