Shown below is a plot of initial rate vs. substrate concentration for an enzyme-substrate reaction that follows Michaelis-Menton kinetics. An analyst developing a quantitative method for the substrate wishes to use a one-point standardization. The standard selected by the analyst, and the corresponding initial rate, are shown on the diagram. Will this one-point standardization give accurate results? Be sure to clearly justify your response.

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accuracy acid-base acid-base titration amino acid back-titration calibration curve chemical reactivity complexation titration concentrations confidence interval derivative titration curve determinate error dilution dimensional analysis end point equivalent weight external standardization extraction graphing indeterminate error interferent ion-exchange chromatography ion-selective electrode ladder diagram mean median method development mixtures non-aqueous precision propagation of error quantitative analysis repeatability reproducibility sampling constant sampling plan selectivity selectivity coefficient solubility standard addition standard deviation standards student-generated data t-test titration curve### Archives

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